One difference in the DNA replication of prokaryotic and eukaryotic cells is that while the DNA of eukaryotic cells is contained in the cell’s nucleus, the DNA of prokaryotic cells is contained in the cell’s cytoplasmic matter.
In eukaryotic cells, the cells split right down the middle and can only occur once per time. In prokaryotic cells, however, the splitting can occur at both ends of the cell.
In eukaryotic cells, the replication process is short and can happen at any stage of the mitotic process. However, in prokaryotic cells, replication can occur only during the synthesis or S-phase.